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p re ss in vitro kinase assay src  (Sino Biological)


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    Sino Biological p re ss in vitro kinase assay src
    P Re Ss In Vitro Kinase Assay Src, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vitro+kinase+assay/SRC%2C+Active/pm41775683-213-5-27
    Average 94 stars, based on 12 article reviews
    p re ss in vitro kinase assay src - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Kinase Assay:

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. The Flag M2 beads pre-loaded with Flag-GFP or Flag-IRE1α proteins were rinsed with kinase assay buffer I (SignalChem, K01–09, 25 mM MOPS pH7.2, 12.5 mM beta-glycerol-phosphate, 25 mM MgCl 2 , 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT) and subjected to in vitro kinase assay in the kinase assay buffer I plus 0.4 mM cold ATP and 1.0 μg GST-ERK2 activated by MEK1 in vitro (SignalChem, M28–10G-20). ..

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. For in vitro kinase assay with recombinant His-IRE1α, 1.0 μg protein purified from Sf9 cells ( 83 ) and recombinant GST-ERK2 protein, the reaction was carried out in the kinase assay buffer I (SignalChem, K01–09) plus 0.4 mM cold ATP for 30 min at 30 °C. .. The reaction was stopped by adding 8 M urea buffer followed by purification of His-IRE1α with Ni-NTA agarose (QIAGEN, 30210).

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..

    Article Title: Nuclear S6K1 Enhances Oncogenic Wnt Signaling by Inducing Wnt/β-Catenin Transcriptional Complex Formation
    Article Snippet: .. Rapamycin, the mTOR specific inhibitor (Calbiochem, 553210, Darmstadt, Germany); PF-4708671, the S6K1 specific inhibitor (Tocris, 4032, Bristol, UK); Wnt-3a, Wnt activator that binds to Frizzled (R&D Systems, 5036-WN, Minneapolis, MN, USA); lithium chloride (LiCl), the GSK-3 inhibitor that acts as a Wnt activator (Sigma Aldrich, L9650, St. Louis, MO, USA); and active p70 S6K1 protein for in vitro kinase assay (SignalChem, R21-10H, Richmond, BC, Canada). .. HEK293T cells were grown in Dulbecco’s modified Eagle medium (DMEM, Welgene, Gyeongsan, Republic of Korea).

    Article Title: Azelnidipine inhibits esophageal squamous cell carcinoma proliferation in vivo and in vitro by targeting MEK1/2
    Article Snippet: .. In vitro kinase assay The active MEK1 and MEK2 (100 ng) were blended by various concentrations of azelnidipine in kinase buffer II (Cat #K02–09; SignalChem, Canada). ..

    Article Title: The mechanism of BUD13 m6A methylation mediated MBNL1-phosphorylation by CDK12 regulating the vasculogenic mimicry in glioblastoma cells
    Article Snippet: .. In vitro kinase assay was performed at 30 °C for 20 min containing 10 μg MBNL1 protein, 5 μg CDK12 kinase (Signalchem, BC, Canada), 50 μL kinase buffer (Cell Signaling Technology, MA, USA), 50 mM ATP (Beyotime Institute of Biotechnology, Jiangsu, China), 5 μCi [γ-32P]-ATP (PerkinElmer, MA, USA). .. Stop the kinase reaction with 1×SDS buffer (Beyotime Institute of Biotechnology, Jiangsu, China), and perform autoradiography after SDS-PAGE electrophoresis.

    In Vitro:

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. The Flag M2 beads pre-loaded with Flag-GFP or Flag-IRE1α proteins were rinsed with kinase assay buffer I (SignalChem, K01–09, 25 mM MOPS pH7.2, 12.5 mM beta-glycerol-phosphate, 25 mM MgCl 2 , 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT) and subjected to in vitro kinase assay in the kinase assay buffer I plus 0.4 mM cold ATP and 1.0 μg GST-ERK2 activated by MEK1 in vitro (SignalChem, M28–10G-20). ..

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. For in vitro kinase assay with recombinant His-IRE1α, 1.0 μg protein purified from Sf9 cells ( 83 ) and recombinant GST-ERK2 protein, the reaction was carried out in the kinase assay buffer I (SignalChem, K01–09) plus 0.4 mM cold ATP for 30 min at 30 °C. .. The reaction was stopped by adding 8 M urea buffer followed by purification of His-IRE1α with Ni-NTA agarose (QIAGEN, 30210).

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..

    Article Title: Nuclear S6K1 Enhances Oncogenic Wnt Signaling by Inducing Wnt/β-Catenin Transcriptional Complex Formation
    Article Snippet: .. Rapamycin, the mTOR specific inhibitor (Calbiochem, 553210, Darmstadt, Germany); PF-4708671, the S6K1 specific inhibitor (Tocris, 4032, Bristol, UK); Wnt-3a, Wnt activator that binds to Frizzled (R&D Systems, 5036-WN, Minneapolis, MN, USA); lithium chloride (LiCl), the GSK-3 inhibitor that acts as a Wnt activator (Sigma Aldrich, L9650, St. Louis, MO, USA); and active p70 S6K1 protein for in vitro kinase assay (SignalChem, R21-10H, Richmond, BC, Canada). .. HEK293T cells were grown in Dulbecco’s modified Eagle medium (DMEM, Welgene, Gyeongsan, Republic of Korea).

    Article Title: Azelnidipine inhibits esophageal squamous cell carcinoma proliferation in vivo and in vitro by targeting MEK1/2
    Article Snippet: .. In vitro kinase assay The active MEK1 and MEK2 (100 ng) were blended by various concentrations of azelnidipine in kinase buffer II (Cat #K02–09; SignalChem, Canada). ..

    Article Title: The mechanism of BUD13 m6A methylation mediated MBNL1-phosphorylation by CDK12 regulating the vasculogenic mimicry in glioblastoma cells
    Article Snippet: .. In vitro kinase assay was performed at 30 °C for 20 min containing 10 μg MBNL1 protein, 5 μg CDK12 kinase (Signalchem, BC, Canada), 50 μL kinase buffer (Cell Signaling Technology, MA, USA), 50 mM ATP (Beyotime Institute of Biotechnology, Jiangsu, China), 5 μCi [γ-32P]-ATP (PerkinElmer, MA, USA). .. Stop the kinase reaction with 1×SDS buffer (Beyotime Institute of Biotechnology, Jiangsu, China), and perform autoradiography after SDS-PAGE electrophoresis.

    Recombinant:

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. For in vitro kinase assay with recombinant His-IRE1α, 1.0 μg protein purified from Sf9 cells ( 83 ) and recombinant GST-ERK2 protein, the reaction was carried out in the kinase assay buffer I (SignalChem, K01–09) plus 0.4 mM cold ATP for 30 min at 30 °C. .. The reaction was stopped by adding 8 M urea buffer followed by purification of His-IRE1α with Ni-NTA agarose (QIAGEN, 30210).

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..

    Purification:

    Article Title: Modulation of the Proteostasis Network Promotes Tumor Resistance to Oncogenic KRAS Inhibitors
    Article Snippet: .. For in vitro kinase assay with recombinant His-IRE1α, 1.0 μg protein purified from Sf9 cells ( 83 ) and recombinant GST-ERK2 protein, the reaction was carried out in the kinase assay buffer I (SignalChem, K01–09) plus 0.4 mM cold ATP for 30 min at 30 °C. .. The reaction was stopped by adding 8 M urea buffer followed by purification of His-IRE1α with Ni-NTA agarose (QIAGEN, 30210).

    Incubation:

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..

    SDS Page:

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..

    Autoradiography:

    Article Title: Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550.
    Article Snippet: .. In vitro kinase assay with recombinant full-length huntingtin and peptides For radiation detection method, 10 ng of PRKACA (SignalChem) was incubated with 5 μg of huntingtin in a buffer containing 50 mM Tris–HCl pH 7.5, 10 mM MgCl2, 0.1 mM EDTA, 2 mM DTT and 2 μCi ATP/[γ -32P]-ATP at 30◦C for 1 h. The reaction was stopped by boiling at 95◦C for 10 min. Phosphorylated huntingtin was analyzed by SDS-PAGE and visualized by autoradiography with a Typhoon FLA 7000 (GE healthcare). ..



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    A – C Wild type ( wt ) B6 ( n = 5) and Stat1 -/- mice ( n = 5) were injected with bleomycin (Bleo) subcutaneously and skin harvested on day 21 for histology ( A ), dermal skin thickness measurement ( B ) and collagen levels assayed by hydroxyproline content ( C ). D – F Stat1 -/- → wt ( n = 7) and wt → wt ( n = 8) bone marrow transplants were analyzed by FACS analysis of peripheral blood immune cells ( D ), then injected with bleomycin as in ( A ) and skin was analyzed for dermal thickness ( E ) and collagen ( F ). G , H Stat1 fl/fl / Pdgfra-Cre ( Stat1 ΔFib ) mice (PBS n = 5, Bleo n = 10) compared to Stat1 fl /fl controls (PBS n = 8, Bleo n = 6) were injected with bleomycin as in ( A ) and analyzed for dermal skin thickness ( G ) and histology ( H ). I, J Wt mice were injected with bleomycin subcutaneously and on the same day treated with the EGFR kinase inhibitor gefitinib (EGFRi) or JAK1/2 inhibitor ruxolitinib p.o. 5 days a week for 3 weeks (PBS n = 8, Bleo+Veh n = 12, Bleo+EGFRi n = 8, Bleo+JAK1/2i n = 7). Skin was analyzed for histology ( I ) and dermal thickness ( J ). Data in ( B , C , E , F , H , J ) are mean ± SD analyzed by two-way ANOVA ( B , C , E , F , H ) or one-way ANOVA ( J ) with Sidak’s multiple comparisons test ( B , C ), uncorrected Fisher’s LSD ( E , F , H ), or Tukey’s multiple comparisons test ( J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: EGFR-STAT1 pathway drives fibrosis initiation in fibroinflammatory skin diseases

    doi: 10.1038/s41467-025-64648-9

    Figure Lengend Snippet: A – C Wild type ( wt ) B6 ( n = 5) and Stat1 -/- mice ( n = 5) were injected with bleomycin (Bleo) subcutaneously and skin harvested on day 21 for histology ( A ), dermal skin thickness measurement ( B ) and collagen levels assayed by hydroxyproline content ( C ). D – F Stat1 -/- → wt ( n = 7) and wt → wt ( n = 8) bone marrow transplants were analyzed by FACS analysis of peripheral blood immune cells ( D ), then injected with bleomycin as in ( A ) and skin was analyzed for dermal thickness ( E ) and collagen ( F ). G , H Stat1 fl/fl / Pdgfra-Cre ( Stat1 ΔFib ) mice (PBS n = 5, Bleo n = 10) compared to Stat1 fl /fl controls (PBS n = 8, Bleo n = 6) were injected with bleomycin as in ( A ) and analyzed for dermal skin thickness ( G ) and histology ( H ). I, J Wt mice were injected with bleomycin subcutaneously and on the same day treated with the EGFR kinase inhibitor gefitinib (EGFRi) or JAK1/2 inhibitor ruxolitinib p.o. 5 days a week for 3 weeks (PBS n = 8, Bleo+Veh n = 12, Bleo+EGFRi n = 8, Bleo+JAK1/2i n = 7). Skin was analyzed for histology ( I ) and dermal thickness ( J ). Data in ( B , C , E , F , H , J ) are mean ± SD analyzed by two-way ANOVA ( B , C , E , F , H ) or one-way ANOVA ( J ) with Sidak’s multiple comparisons test ( B , C ), uncorrected Fisher’s LSD ( E , F , H ), or Tukey’s multiple comparisons test ( J ) (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Source data are provided as a Source Data file.

    Article Snippet: The EGFR in vitro kinase assay (BPS Bioscience 40321) was performed by incubating recombinant human STAT1 and recombinant human EGFR kinase in the kinase buffer (provided with the kit) supplemented with 100 μM ATP and 2 mM DTT.

    Techniques: Injection

    A – F Western blots of human foreskin fibroblasts (HFF) treated with EGFR ligands 100 ng/ml, IFNA2 1000 U/ml, or IFNG 1000 U/ml for 5 min unless otherwise noted. A STAT1, AKT and ERK phosphorylation in response to each EGFR ligand. B Time course of HFF treated with IFNA2 or TGFA. C HFF pretreated with or without gefitinib 5 μM for 30 min before addition of TGFA or EREG. D HFF treated with deucravacitinib (TYK2i) 0, 50 nM and 250 nM for 2 h before addition of IFNA2 or TGFA. E HFF treated with ruxolitinib (JAK1/2i) 0, 50 nM and 250 nM for 2 h before addition of IFNA2, IFNG or TGFA. F HFF treated with combined TYK2i and JAK1/2i before IFNA2 or TGFA. G In vitro kinase assay using recombinant EGFR kinase domain and recombinant STAT1 with increasing concentrations of gefitinib (EGFRi) analyzed for pSTAT1 (pY701) by western blot ( n = 3 per group). Quantification of the signal done by densitometry. H , I Proximity ligation assay (PLA) of HFF with and without TGFA 100 ng/ml for 5 min. Single antibody PLA was used as negative controls to account for any background signal from each antibody. PLA signal was quantified (I) as dots per cell in each 40x field across 5 replicates. Data in ( G , I ) are mean ± SD analyzed by one-way ANOVA with Tukey multiple-comparisons test (** p < 0.01, **** p < 0.0001, ns: not significant). All panels are representative of at least two independent experiments. GAPDH was used as the loading control in all western blots ( A – F ). Diagram of human icon created in BioRender. Odell, I. (2025) https://BioRender.com/emkz6wu . Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: EGFR-STAT1 pathway drives fibrosis initiation in fibroinflammatory skin diseases

    doi: 10.1038/s41467-025-64648-9

    Figure Lengend Snippet: A – F Western blots of human foreskin fibroblasts (HFF) treated with EGFR ligands 100 ng/ml, IFNA2 1000 U/ml, or IFNG 1000 U/ml for 5 min unless otherwise noted. A STAT1, AKT and ERK phosphorylation in response to each EGFR ligand. B Time course of HFF treated with IFNA2 or TGFA. C HFF pretreated with or without gefitinib 5 μM for 30 min before addition of TGFA or EREG. D HFF treated with deucravacitinib (TYK2i) 0, 50 nM and 250 nM for 2 h before addition of IFNA2 or TGFA. E HFF treated with ruxolitinib (JAK1/2i) 0, 50 nM and 250 nM for 2 h before addition of IFNA2, IFNG or TGFA. F HFF treated with combined TYK2i and JAK1/2i before IFNA2 or TGFA. G In vitro kinase assay using recombinant EGFR kinase domain and recombinant STAT1 with increasing concentrations of gefitinib (EGFRi) analyzed for pSTAT1 (pY701) by western blot ( n = 3 per group). Quantification of the signal done by densitometry. H , I Proximity ligation assay (PLA) of HFF with and without TGFA 100 ng/ml for 5 min. Single antibody PLA was used as negative controls to account for any background signal from each antibody. PLA signal was quantified (I) as dots per cell in each 40x field across 5 replicates. Data in ( G , I ) are mean ± SD analyzed by one-way ANOVA with Tukey multiple-comparisons test (** p < 0.01, **** p < 0.0001, ns: not significant). All panels are representative of at least two independent experiments. GAPDH was used as the loading control in all western blots ( A – F ). Diagram of human icon created in BioRender. Odell, I. (2025) https://BioRender.com/emkz6wu . Source data are provided as a Source Data file.

    Article Snippet: The EGFR in vitro kinase assay (BPS Bioscience 40321) was performed by incubating recombinant human STAT1 and recombinant human EGFR kinase in the kinase buffer (provided with the kit) supplemented with 100 μM ATP and 2 mM DTT.

    Techniques: Western Blot, Phospho-proteomics, In Vitro, Kinase Assay, Recombinant, Proximity Ligation Assay, Control

    A Time course of mNDF treated with TGFA 100 ng/ml compared to non-treated (NT) controls analyzed by qPCR, expression levels shown in heatmap. B Diagram of promoter regions and first three exons (blue boxes, not drawn to scale) of STAT1, overlapping EGR1/SP1, ELK4 and FOS binding sites. C–F Western blot ( C ) and qPCR (D – F , n = 4 per group ) of HFF pretreated with termuterkib (ERKi) or AZD8330 (MEKi) 100 nM prior to TGFA 100 ng/ml. G – K HFF pretreated with fludarabine (STATi) 100μM and analyzed by qPCR (G , I – K) and western blot ( H ) (G, n = 8 per group; I – K , n = 4 per group). L Diagram of the regulatory network between EGFR, ERK, EGR1 and STAT1 driving fibrotic gene expression. Mouse and human icons indicate from what organism the fibroblasts were isolated. Diagrams created in Biorender. Odell, I. (2025) https://BioRender.com/th7ial3 , https://BioRender.com/emkz6wu , https://BioRender.com/w2lhyzb . Data in ( D – F , I – K ) are mean ± SD analyzed by one-way ANOVA with Tukey multiple-comparisons test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data in ( G ) is mean ± SD analyzed by two-tailed unpaired t -Test (** p < 0.01). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: EGFR-STAT1 pathway drives fibrosis initiation in fibroinflammatory skin diseases

    doi: 10.1038/s41467-025-64648-9

    Figure Lengend Snippet: A Time course of mNDF treated with TGFA 100 ng/ml compared to non-treated (NT) controls analyzed by qPCR, expression levels shown in heatmap. B Diagram of promoter regions and first three exons (blue boxes, not drawn to scale) of STAT1, overlapping EGR1/SP1, ELK4 and FOS binding sites. C–F Western blot ( C ) and qPCR (D – F , n = 4 per group ) of HFF pretreated with termuterkib (ERKi) or AZD8330 (MEKi) 100 nM prior to TGFA 100 ng/ml. G – K HFF pretreated with fludarabine (STATi) 100μM and analyzed by qPCR (G , I – K) and western blot ( H ) (G, n = 8 per group; I – K , n = 4 per group). L Diagram of the regulatory network between EGFR, ERK, EGR1 and STAT1 driving fibrotic gene expression. Mouse and human icons indicate from what organism the fibroblasts were isolated. Diagrams created in Biorender. Odell, I. (2025) https://BioRender.com/th7ial3 , https://BioRender.com/emkz6wu , https://BioRender.com/w2lhyzb . Data in ( D – F , I – K ) are mean ± SD analyzed by one-way ANOVA with Tukey multiple-comparisons test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: not significant). Data in ( G ) is mean ± SD analyzed by two-tailed unpaired t -Test (** p < 0.01). Source data are provided as a Source Data file.

    Article Snippet: The EGFR in vitro kinase assay (BPS Bioscience 40321) was performed by incubating recombinant human STAT1 and recombinant human EGFR kinase in the kinase buffer (provided with the kit) supplemented with 100 μM ATP and 2 mM DTT.

    Techniques: Expressing, Binding Assay, Western Blot, Gene Expression, Isolation, Two Tailed Test

    Activated keratinocytes defined by expression of S100A8 and stress keratins express high affinity EGFR ligands including TGFA. TGFA and possibly other high affinity ligands bind EGFR on fibroblasts, which phosphorylates STAT1 to induce a fibrotic expression profile. STAT1 acts as a master regulator of fibrosis genes independent of type I interferon signaling. Created in BioRender. Odell, I. (2025) https://BioRender.com/7b1sqwc .

    Journal: Nature Communications

    Article Title: EGFR-STAT1 pathway drives fibrosis initiation in fibroinflammatory skin diseases

    doi: 10.1038/s41467-025-64648-9

    Figure Lengend Snippet: Activated keratinocytes defined by expression of S100A8 and stress keratins express high affinity EGFR ligands including TGFA. TGFA and possibly other high affinity ligands bind EGFR on fibroblasts, which phosphorylates STAT1 to induce a fibrotic expression profile. STAT1 acts as a master regulator of fibrosis genes independent of type I interferon signaling. Created in BioRender. Odell, I. (2025) https://BioRender.com/7b1sqwc .

    Article Snippet: The EGFR in vitro kinase assay (BPS Bioscience 40321) was performed by incubating recombinant human STAT1 and recombinant human EGFR kinase in the kinase buffer (provided with the kit) supplemented with 100 μM ATP and 2 mM DTT.

    Techniques: Expressing